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The location of the center of gravity (CG) can indeed move aft during flight, particularly as fuel is consumed from forward fuel tanks. This scenario occurs because as fuel is burned, it typically reduces the overall weight and shifts the balance of the aircraft toward the rear. Given that fuel is often stored in tanks located towards the front of the aircraft, depleting these fuel tanks during initial ascent causes the CG to shift rearward.

This shifting of the CG can significantly affect the aircraft’s handling characteristics and requires careful monitoring to ensure that the aircraft remains within its operational limits. Pilots must be aware of this dynamic change in CG position during flight when planning for fuel consumption, as it will influence the aircraft's pitch and stability.

In contrast, adding more cargo would generally shift the CG forward, while adjusting trim settings does not affect the CG location itself but rather modifies the aerodynamic forces acting on the aircraft. Additionally, during takeoff, the CG ideally should be within acceptable limits for stable flight but is not necessarily moving aft at that point.